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Agnieszka Pekala-Safinska, Ph.D., D.Sc., D.V.M.,
Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland
Halyna Tkachenko, Ph.D., D.Sc.,
Zbigniew Osadowski, Ph.D., D.Sc.,
Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland
Lyudmyla Buyun, Ph.D., D.Sc.,
M. M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
Vitaliy Honcharenko, Ph.D., Associate Professor
Ivan Franko Lviv National University, Lviv, Ukraine
Andriy Prokopiv, Ph.D., Associate Professor
Botanic Garden of Ivan Franko Lviv National University, Lviv, Ukraine


Keywords: Ficus lyrata, Ficus lyrata ‘Bambino’, Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida, antimicrobial activity, disc diffusion technique, ethanolic extracts.


The aim of the present study was to assess the antibacterial efficacy of ethanolic extracts derived from F. lyrata and its cultivar F. lyrata ‘Bambino’against three Aeromonas strains (Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida). TheleavesofplantswerecollectedinM. M. GryshkoNationalBotanicGarden (NBG, Kyiv, Ukraine) andBotanicGardenofIvanFrankoLvivNationalUniversity (Lviv, Ukraine). Freshly collected leaves were weighed and homogenized in 96 % ethanol (in proportion 1:10) at room temperature. Three Aeromonas strains: Aeromonas sobria (K825) and Aeromonas hydrophila (K886), as well as Aeromonas salmonicida subsp. salmonicida (St30), originated from freshwater fish species such as common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum), respectively, were isolated in Department of Fish Diseases, The National Veterinary Research Institute in Pulawy (Poland). Bacteria were collected from fish exhibiting clinical disorders. Our results demonstrated that three Aeromonas strains (Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida) were resistant to ethanolic extract derived from F. lyrata. The inhibition zone diameters were (9.50±0.33 mm), (9.38±0.38 mm), and (9.5±0.5 mm) for Aeromonas sobria, Aeromonas hydrophila and Aeromonas salmonicida subsp. salmonicida (St30), respectively. F. lyrata ‘Bambino’ extract exhibited the intermediate activity against Aeromonas sobria (inhibition zone diameter was 12±0.73 mm), while Aeromonas hydrophila and Aeromonas salmonicida subsp. salmonicida (St30) were resistant (inhibition zone diameters were 9.18±0.54 mm and 9.13±0.44 mm). These results pave the way for the possible development of natural additives to replace synthetic ones. Therefore, further investigations for the isolation of active constituents and their pharmacological evaluation as well as in vitro and in vivo study are required.


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