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Lyudmyla Buyun, Ph.D., D.Sc.,
Igor Kharchenko, Ph.D.
Myroslava Maryniuk, Post-graduate student
M. M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
Halyna Tkachenko, Ph.D., D.Sc.,
Zbigniew Osadowski, Ph.D., D.Sc.,
Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Poland


Keywords: rainbow trout (Oncorhynchus mykiss Walbaum), muscle tissue, Camellia japonica L., cultivars, oxidatively modified proteins.


In this study, we investigated the antioxidative activity of plant extracts by the measurement of aldehydic and ketonic derivatives of oxidatively modified proteins. In our study, Camellia japonica L. 'Kramer’s Supreme', Camellia japonica 'C. M. Wilson', Camellia japonica 'La Pace', Camellia japonica ‘Benikarako’, Camellia japonica ‘Fanny Bolis’ were protectively effective extracts caused the decrease of protein oxidative damage in the muscle tissue of rainbow trout. The leaves of various Camellia japonica cultivars, cultivated under glasshouse conditions, were sampled at M. M. Gryshko National Botanical Garden (NBG), National Academy of Sciences of Ukraine. Freshly collected leaves were washed, weighed, crushed, and homogenized in 100 mM phosphate buffer (pH 7.2) (in proportion 1:19, w/w) at room temperature. The extracts were then filtered and investigated. Clinically healthy rainbow trout with a mean body mass of 80-120 g were used in the experiments. The muscle tissue samples were homogenized in ice-cold buffer (100 mM Tris-HCl, pH 7.2) using a glass homogenizer immersed in ice water bath. Homogenates were centrifuged at 3,000 g for 15 min at 4°C. The supernatant of the muscle tissue was used to incubate with extracts of various cultivars of C. japonica (in a ratio 19:1) at room temperature. The positive control group (trout muscle tissue) was incubated with 100 mM Tris-HCl buffer (pH 7.2) (in a ratio 19:1). The incubation time was 2 hours. When muscle tissue was incubated with leaf extracts of various C. japonica cultivars, the aldehydic derivatives level was ranged to the value of the control group. Among the six plant extracts, C. japonica 'La Pace' exhibited the highest inhibitory effect (the decrease of ketonic derivatives was 28.2%, p<0.05 compared to the control group). The level of ketonic derivatives of oxidatively modified proteins in the sample with C. japonica 'Mrs. Lyman Clarke' was increased by 2.8% (p>0.05) compared to controls. The least level of ketonic derivatives is attributed to C. japonica 'La Pace' extract. Therefore, Therefore, Camellia japonica may be used as an antioxidant agent in aquaculture as it can be easily obtained and is not expensive. Furthermore, the use of such plant products as antioxidants and immunostimulants in aquaculture systems may also have environmental value because of their biodegradability.


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