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Ladyka Volodymyr, Doctor of Agricultural Sciences, Academician of NAAS
https://orcid.org/0000-0001-6748-7616
Pavlenko Yuliya, PhD, Associate Professor
https://orcid.org/0000-0002-4128-122X
Sumy National Agrarian University
Drevytska TetianaMSc PhD
https://orcid.org/0000-0002-3192-4682
Dosenko Victor, MD PhD DrSc Professor
https://orcid.org/0000-0002-6919-7724
Bogomoletz Institute of Physiology
Skliarenko Yuri, Doctor of Agricultural Sciences
https://orcid.org/0000-0002-6579-2382
Ukraines North-east Agriculture Research Institute

 

DOI https://doi.org/10.32900/2312-8402-2021-126-62-69

Keywords: fat content, protein content, β-casein, genotype, allele

Abstract

In the breeding herds of the Ukrainian Black-and-White Dairy breed, the research was conducted to study the polymorphism of β-casein gene and its effect on the qualitative composition of cow's milk. Genotyping of 146 heads of cattle was carried out. Determination of β-casein gene polymorphism was performed in the genetic laboratory of Bohomolets Institute of Physiology. The TagMan@Genotyping system and a set of primers and probes were used for allelic discrimination.

It was found that the frequencies of alleles A1 (0.421) and A2 (0.579) in the locus of β-casein gene were different, the latter being higher. Consequently, the frequencies of genotypes A1A1, A1A2 and A2A2 were different, 25%, 35% and 40% respectively. According to genetic and statistical analysis, there was an excess of homozygous variants of A1A1 and A2A2 in the β-casein locus, and a lack of heterozygous A1A2.

There was a difference in the quality characteristics of milk between animals of different genotypes. The content of individual milk constituents did not vary equally depending on the β-casein genotype, but this difference was statistically insignificant.

The average content of constituents in the milk of cows corresponded to the breed standards in terms of fat content and was slightly inferior to it in terms of protein content. Cows of Ukrainian Black-and-White Dairy breed with heterozygous A1A2 genotype exceeded others in terms of protein, lactose and dried skimmed milk remainder. Homozygous genotypes A1A1 and A2A2 had higher fat content in milk than heterozygotes. As there is no statistically significant difference in the main milk constituents (fat, protein) between animals of different genotypes for β-casein, the formation of herds with A2A2 genotype for β-casein will not adversely affect the productive traits of cows and thus ensure sufficient quality indicators of milk of new herd types. Livestock breeders need to pay some attention to selection and technological measures to increase the protein content in milk.

 References

  1. Marzanov, N. S., Devrishov, D. A., Marzanova, S. N., Abylkasymov, D. A., Konovalova, N. V., Libet, I. S. (2020). Characterization of Russian dairy cattle breeds by the occurrence of genotypes and alleles at the beta-casein locus [Characteristics of the rossesky molded porosity of a large horned cattle for the inspection of genotypes and alleles in the beta-casein location.]. Veterinary Science Animal Science Biotechnology – Veterinarian zootechnia biotechnology. Moskva. no. 1. pp. 47-52. [in Russian]
  2. Amalfitano, N., Cipolat-Gotet, C., Cecchinato, A., Malacarne, M., Summer, A., Bittante, G. (2018). Milk protein fractions strongly affect the patterns of coagulation, curd firming, and syneresis. Journal of Dairy Science. 102, 2903–2917. Retrieved from https://doi.org/10.3168/jds.2018-15524
  3. Bentivoglio, D., Finco, A., Bucci, G., Staffolani, G. (2020). Is There a Promising Market for the A2 Milk? Analysis of Italian Consumer Preferences. Sustainability. 12, 17, 6763. Retrieved from https://doi.org/10.3390/su12176763
  4. Fuerer, C., Jenni, R., Cardinaux, L., Andetsion, F., Wagnière, S., Moulin, J., Affolter M. (2020). Protein fingerprinting and quantification of β-casein variants by ultraperformance liquid chromatography–high-resolution mass spectrometry. Journal of Dairy Science, 103(2), 1193–1207. Retrieved from https://doi.org/10.3168/jds.2019-16273
  5. Gigliotia, R., Gutmanisa, G., Katikia, L., Okinob, C., Oliveirab, M., Filhoa, A. (2020). New high-sensitive rhAmp method for A1 allele detection in A2 milk samples. Food Chemistry, 313, 1-7. Retrieved from https://doi.org/10.1016/j.foodchem.2020.126167
  6. Gustavsson, F., Buitenhuis, A., Johansson, M., Bertelsen, H., Glantz, M., Poulsen, N. (2013). Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. Journal of Dairy Science. 97(6), 3866–3877.
  7. Louise, S., Jackeline, S., Marisa, S., Raphael, B., Camargo, G. (2021). Do non-bovine domestic animals produce A2 milk?: an in silico analysis. Animal Biotechnology.  Retrieved from https://doi.org/10.1080/10495398.2021.1935982
  8. Mayer, H., Lenz, K., Halbauer, E. (2021). “A2 milk” authentication using isoelectric focusing and different PCR techniques. Food Research International. 147, 2-9. Retrieved from https://doi.org/10.1016/j.foodres.2021.110523
  9. Miluchová, M., Gábor, M., Candrák, J., Trakovická, A., Candráková, K. (2018). Association of HindIII-polymorphism in kappa-casein gene with milk, fat and protein yield in holstein cattle. ActaBiochimicaPolonica. 65, 3, 403–407. Retrieved from https://doi.org/10.18388/abp. 2017_2313
  10. O'Callaghan, T. (2020). An overview of the A1/A2 milk hypothesis. DairyNutritionforum. 12 (2), 1-4. Retrieved from file:///C:/Users/user/Downloads/DNForumA1A2milk2020pdf.pdf
  11. Parashar, A., Saini, R. (2020). A1 milk and its controversy-areview. International Journal of Bioassays. 4, 12, 4611-4619.
  12. Sebastiani, C., Arcangeli, C., Ciullo, M., Torricelli, M., Cinti, G., Fisichella, S., Biagetti, M. (2020). Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals. 10, 2, 2-7. Retrieved from https://doi.org/10.3390/ani10020252

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